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Design is optimized for reducing the time for gene amplification.
Sample is loaded in flat and wide chamber so that heat is efficiently delivered to the sample.
Well volume is 10μl only, which helps fast heating and cooling.
Bottom surface of chip is made of thin film for efficient heat transfer.
Contamination free and transformation free.
Options are available for thermal cycling and real-time analysis.
Rapi:chip™ was developed from the understanding of the challenges in routine PCR.
We found the protocols duration could be dramatically reduced when efficient heat transfer is made during the thermal cycling process.
By designing a unique 3-dimensional polymer chip where the bottom surface is made of a thin film and precisely bonded with a polymer body, ultra-fast thermal cycling became possible.
Rapi:chip™ is supplied in two formats. The QPCR version of the chip has 10 wells and the top of each well is open for real-time fluorescence measurement.
Rapi:chip/16™ is a version which can accommodate 16 samples and is ideal for amplification of standard PCR. It does not have the capability for analysis of a fluorescence signal.
The wings on either side of the chip are used for easy handling with fingers or tweezers.
The Well numbers are printed on the upper surface of the chip for easy identification.
Pipette tips fit in the apertures so that sample and reagent can be loaded or collected easily.
HOW TO LOAD SAMPLES
STEP 1. Prepare sample according to suggested protocol (10μl).
STEP 2. Using micropipette, aspirate prepared sample mixture (10μl).
STEP 3. Place pipette tip into the inlet hole of Rapi:chip™ and dispense whole volume of aspirated sample.
STEP 4. Seal entire holes using enclosed sealing tape. Scrub the surface of sealed area to secure sealing.